CN102875663B - Purification method of lixisenatide - Google Patents

Abstract

The invention relates to a purification method of lixisenatide, which comprises the following steps: step 1, purifying crude peptide of lixisenatide by high performance liquid chromatography, wherein the high performance liquid chromatography adopts phenyl silane bonded silica gel as a stationary phase, adopts a methanol/phosphate buffered solution of D-tartrate as a mobile phase A, and adopts acetonitrile as a mobile phase B for gradient elution; step 2, performing salt conversion purification of the fractions obtained in step 1 by high performance liquid chromatography, wherein the high performance liquid chromatography adopts alkyl silane bonded silica gel as a stationary phase, adopts a glacial acetic acid solution as a mobile phase A, and adopts acetonitrile as a mobile phase B for gradient elution; step 3, collecting the solution, and performing freeze-drying.

Description

The purification process of lixisenatide

Technical field

The invention belongs to pharmaceutical chemistry field, be specifically related to the purification process of lixisenatide.

Background technology

Diabetes are a kind of global height morbidities, and it is mainly divided into I type and II type, and the latter accounts for the more than 90% of diabetic subject's sum.

Usually treat patients with NIDDM by glucagon-like peptide 1 (GLP-1) receptor antagonist lixisenatide (Lixisenatide) combined foundation pancreas islet, can significantly improve its glycemic control, and the control that improves its postprandial blood sugar (PPG).

The long-chain polypeptide that lixisenatide is made up of 43 amino-acid residues, and in peptide sequence just like the easy isomerized amino acid and cause containing a lot of isomer impurities in the thick peptide of lixisenatide in Solid-phase Polypeptide chemosynthesis process such as Ser, His, Pro.Therefore the separation and purification of lixisenatide becomes the technological difficulties in this medicine preparation technology, and the purifying of the lixisenatide of especially extensive preparation has become one of bottleneck of its industrialization of restriction.

Summary of the invention

The object of this invention is to provide a kind of purification process of lixisenatide, especially pass through Solid-phase Polypeptide chemical synthesis process and the purification process of the extensive lixisenatide of preparing.

The purification process of lixisenatide of the present invention comprises the following steps:

Step 1: adopt high performance liquid chromatography (HPLC) method to carry out purifying to the thick peptide of lixisenatide, described HPLC method is take phenyl silane bonded silica gel as stationary phase, take the methyl alcohol/phosphate buffer soln of D-tartrate as mobile phase A phase, carry out mutually gradient elution take acetonitrile as Mobile phase B;

Step 2: the cut that adopts HPLC method to obtain step 1 turns salt purifying, and described HPLC method, take alkyl silane bonded silica gel as stationary phase, take glacial acetic acid solution as mobile phase A phase, is carried out gradient elution take acetonitrile as Mobile phase B mutually;

Step 3: collect solution freeze-drying.

The present invention is by adopting take phenyl silane bonded silica gel as stationary phase, in moving phase, add the HPLC method purifying of D-tartrate and proton donor methyl alcohol, can disposable isomer impurities in thick lixisenatide peptide be separated well and be removed with other difficult separating impurity.Then utilize Reversed phase HPLC method to change into acetate API, improved yield and the purity of product.In addition, this purification process is easy and simple to handle, is conducive to realize the purifying of lixisenatide prepared by mass-producing.

Accompanying drawing explanation

Color atlas after lixisenatide purifying in Fig. 1 embodiment 1.

Embodiment

The purification process of lixisenatide of the present invention comprises the following steps:

Step 1: adopt HPLC method to carry out purifying to the thick peptide of lixisenatide, described HPLC method is take phenyl silane bonded silica gel as stationary phase, take the methyl alcohol/phosphate buffer soln of D-tartrate as mobile phase A phase, carries out gradient elution mutually take acetonitrile as Mobile phase B;

Step 2: the cut that adopts HPLC method to obtain step 1 turns salt purifying, and described HPLC method, take alkyl silane bonded silica gel as stationary phase, take glacial acetic acid solution as mobile phase A phase, is carried out gradient elution take acetonitrile as Mobile phase B mutually;

Step 3: collect solution freeze-drying.

The volumetric molar concentration of the D-tartrate of the mobile phase A of the HPLC method in described step 1 in is mutually preferably 10mM ~ 50mM.

The D-tartrate of the mobile phase A of the HPLC method in described step 1 in is mutually preferably one or more and is selected from the D-tartrate of D-sodium tartrate, D-soluble tartrate, D-ammonium tartrate.

The phosphatic volumetric molar concentration of the mobile phase A of the HPLC method in described step 1 in is mutually preferably 20mM ~ 50mM.

The phosphoric acid salt of the mobile phase A of the HPLC method in described step 1 in is mutually preferably one or more and is selected from the phosphoric acid salt of sodium phosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, potassiumphosphate, dipotassium hydrogen phosphate, potassium primary phosphate, ammonium phosphate or primary ammonium phosphate.

The pH of the mobile phase A phase solution of the HPLC method in described step 1 is preferably 2.0 ~ 4.0.

The volume ratio that the methyl alcohol of the mobile phase A of the HPLC method in described step 1 in mutually accounts for whole mobile phase A phase is preferably 10 ~ 20%(v/v).

The gradient of the HPLC method in described step 1 according to the per-cent of mobile phase A phase is $(75~85)\%A\stackrel{35\min }{\→}(55~65)\%A,$ Can suitably adjust according to retention time.

The concentration expressed in percentage by volume of the glacial acetic acid aqueous solution of the mobile phase A of the HPLC method in described step 2 in is mutually preferably 0.1 ~ 0.3%(v/v).

The stationary phase of the HPLC method in described step 2 is preferably eight alkyl silane bonded silica gels, six alkyl silane bonded silica gels or tetraalkyl silane group silica gel.

The gradient of the HPLC method in described step 2 is $95\%A+5\%B$ $\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B\stackrel{20\min }{\→}65\%A+35\%B$ Can suitably adjust according to retention time.

Purification process of the present invention is simple to operate, thereby makes running cost low.In addition, lixisenatide yield in purification process of the present invention is high, thereby be suitable for the large-scale industrialized production of lixisenatide, and the purified lixisenatide purity obtaining is high, foreign matter content is low, therefore there is considerable economical and practical value and application prospect widely.

Embodiment

The present invention is described in detail by the following examples.

In following examples, the mensuration of lixisenatide content is carried out according to high performance liquid chromatography (two appendix V D of Chinese Pharmacopoeia version in 2010).

Chromatographic condition and system suitability

Chromatographic column used is made weighting agent with phenyl silane bonded silica gel, and chromatographic column specification is 4.6*250mm, and the particle diameter of the phenyl silane bonded silica gel of filling is 5 μ m; With 0.1mol/L ammonium phosphate damping fluid (get primary ammonium phosphate 11.5g, add water to 1000ml, with phosphorus acid for adjusting pH be 2.5), for mobile phase A phase, take trifluoroacetic acid aqueous solution as Mobile phase B phase, according to the form below 1 carries out gradient elution; Flow velocity is 1.0ml/ minute, and detection wavelength is 220nm, and column temperature is 50 ℃; Number of theoretical plate calculates and should be not less than 5000 by lixisenatide peak.

The measuring method of lixisenatide content

Accurately take appropriate lixisenatide crude product, add water and make solution containing 2.0mg/ml lixisenatide crude product as need testing solution, separately accurately take appropriate lixisenatide reference substance, add water and make the solution product solution in contrast containing 1.0mg/ml lixisenatide reference substance; Get the each 20 μ l of reference substance solution and need testing solution injection liquid chromatography respectively, record color atlas, calculate by external standard method with peak area, obtain the content of lixisenatide.

Gradient in the chromatography of table 1 lixisenatide assay

Embodiment 1

Thick lixisenatide peptide 4.0g(is contained to lixisenatide 1.08g) with purified water 200ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 20% methyl alcohol/80% aqueous solution (v/v) of 10mM D-ammonium tartrate and 30mM primary ammonium phosphate, adjusting pH with phosphoric acid is 2.0.

Mobile phase A phase process for preparation: take 18.4g D-ammonium tartrate and 34.5g primary ammonium phosphate, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, adds purified water to 10L scale after adding 2L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 2.0.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $82\%A+18\%B\stackrel{35\min }{\→}62\%A+38\%B$

Applied sample amount: 2.0g (100ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C8 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.10% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}65\%A+35\%B$

Loading volume: 200ml.

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.66g.Purity 98.47%, each foreign matter content is all less than 0.5%.Purification yield 61.1%(is with lixisenatide cubage in crude product), total recovery 16.5%.The results are shown in Figure 1, wherein X-coordinate representative in minute working time, ordinate zou represents peak height.Statistics is in table 2.

The chromatogram detected result of table 2 lixisenatide solid essence peptide

Embodiment 2

Thick lixisenatide peptide 6.0g(is contained to lixisenatide 1.62g) with purified water 300ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 15% methyl alcohol/85% aqueous solution (v/v) of 40mM D-sodium tartrate and 20mM primary ammonium phosphate, adjusting pH with phosphoric acid is 3.0.

Mobile phase A phase process for preparation: take 92.0g D-sodium tartrate and 23.0g primary ammonium phosphate, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, adds purified water to 10L scale after adding 1.5L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 3.0.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $79\%A+21\%B\stackrel{35\min }{\→}59\%A+41\%B$

Applied sample amount: 3.0g (150ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C8 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.15% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}65\%A+35\%B$

Loading volume: 200ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.93g.Purity 98.62%, each foreign matter content is all less than 0.5%.Purification yield 57.4%(is with lixisenatide cubage in crude product), total recovery 15.5%.

Embodiment 3

Thick lixisenatide peptide 6.0g(is contained to lixisenatide 1.62g) with purified water 300ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 30mM D-soluble tartrate and 40mM potassium primary phosphate, adjusting pH with phosphoric acid is 2.5.

Mobile phase A phase process for preparation: take 56.1g D-soluble tartrate and 54.4g potassium primary phosphate, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, adds purified water to 10L scale after adding 1.0L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 2.5.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $80\%A+20\%B\stackrel{35\min }{\→}60\%A+40\%B$

Applied sample amount: 3.0g (150ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C4 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.3% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}70\%A+30\%B$

Loading volume: 250ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.98g.Purity 98.52%, each foreign matter content is all less than 0.5%.Purification yield 60.5%(is with lixisenatide cubage in crude product), total recovery 16.3%.

Embodiment 4

Thick lixisenatide peptide 4.0g(is contained to lixisenatide 1.08g) with purified water 200ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 50mM D-sodium tartrate and 50mM Sodium phosphate dibasic, adjusting pH with phosphoric acid is 3.5.

Mobile phase A phase process for preparation: take 115g D-sodium tartrate and 179g Sodium phosphate dibasic, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, adds purified water to 10L scale after adding 1.0L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 3.5.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $78\%A+22\%B\stackrel{35\min }{\→}58\%A+42\%B$

Applied sample amount: 2.0g (100ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C6 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.20% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}68\%A+32\%B$

Loading volume: 200ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.67g.Purity 98.60%, each foreign matter content is all less than 0.5%.Purification yield 62.0%(is with lixisenatide cubage in crude product), total recovery 16.8%.

Embodiment 5

Thick lixisenatide peptide 4.0g(is contained to lixisenatide 1.08g) with purified water 200ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 20mM D-sodium tartrate and 30mM Sodium phosphate dibasic, adjusting pH with phosphoric acid is 4.0.

Mobile phase A phase process for preparation: take 46.0g D-sodium tartrate and 42.6g Sodium phosphate dibasic, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, adds purified water to 10L scale after adding 1.0L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 4.0.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $77\%A+23\%B\stackrel{35\min }{\→}57\%A+43\%B$

Applied sample amount: 2.0g (100ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C4 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.20% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}70\%A+30\%B$

Loading volume: 200ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.65g.Purity 98.52%, each foreign matter content is all less than 0.5%.Purification yield 60.2%(is with lixisenatide cubage in crude product), total recovery 16.3%.

Embodiment 6

Thick lixisenatide peptide 4.0g(is contained to lixisenatide 1.08g) with purified water 200ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 10mM D-sodium tartrate, 10mM D-ammonium tartrate and 10mM SODIUM PHOSPHATE, MONOBASIC and 10mM primary ammonium phosphate, adjusting pH with phosphoric acid is 2.5.

Mobile phase A phase process for preparation: take 23.0g D-sodium tartrate, 18.4g D-ammonium tartrate and 12.0g SODIUM PHOSPHATE, MONOBASIC and 11.5g primary ammonium phosphate, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 10L serum bottle, after adding 1.0L Chromatographic Pure Methanol, add purified water to 10L scale, adjusting pH with phosphoric acid is 2.5.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $77\%A+23\%B\stackrel{35\min }{\→}57\%A+43\%B$

Applied sample amount: 2.0g (100ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Waters 2545

Chromatographic column: 50 × 250mm, in-built anti-phase C4 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 80ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.20% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}70\%A+30\%B$

Loading volume: 200ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 20ml.

After freeze-drying, obtain white powder solid essence peptide 0.65g.Purity 98.52%, each foreign matter content is all less than 0.5%.Purification yield 60.2%(is with lixisenatide cubage in crude product), total recovery 16.3%.

Embodiment 7

Thick lixisenatide peptide 20g(is contained to lixisenatide 5.70g) with purified water 500ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Novasep LC150

Chromatographic column: 100 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 250ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 20mM D-sodium tartrate and 20mM SODIUM PHOSPHATE, MONOBASIC, adjusting pH with phosphoric acid is 2.0.

Mobile phase A phase process for preparation: take 92.0g D-sodium tartrate and 48.0g SODIUM PHOSPHATE, MONOBASIC, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 20L serum bottle, adds purified water to 20L scale after adding 2.0L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 2.0.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $82\%A+18\%B\stackrel{35\min }{\→}62\%A+38\%B$

Applied sample amount: 10.0g (250ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Novasep LC150

Chromatographic column: 100 × 250mm, in-built anti-phase C8 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 250ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.1% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}65\%A+35\%B$

Loading volume: 500ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 50ml.

After freeze-drying, obtain white powder solid essence peptide 3.31g.Purity 98.52%, each foreign matter content is all less than 0.5%.Purification yield 58.1%(is with lixisenatide cubage in crude product), total recovery 16.6%.

Embodiment 8

Thick lixisenatide peptide 200g(is contained to lixisenatide 57.0g) with purified water 5000ml dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Novasep LC300

Chromatographic column: 300 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 2000ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 30mM D-sodium tartrate and 30mM Sodium phosphate dibasic, adjusting pH with phosphoric acid is 2.5.

Mobile phase A phase process for preparation: take 1035g D-sodium tartrate and 639g Sodium phosphate dibasic, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 150L container for storing liquid, adds purified water to 150L scale after adding 15L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 2.5.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $80\%A+20\%B\stackrel{35\min }{\→}60\%A+40\%B$

Applied sample amount: 100.0g (2500ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Sample introduction at twice, repeats above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Novasep LC300

Chromatographic column: 300 × 250mm, in-built anti-phase C8 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 2000ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.2% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}65\%A+35\%B$

Loading volume: 500ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 450ml.

After freeze-drying, obtain white powder solid essence peptide 35.3g.Purity 98.59%, each foreign matter content is all less than 0.5%.Purification yield 61.9%(is with lixisenatide cubage in crude product), total recovery 17.4%.

Embodiment 9

Thick lixisenatide peptide 4000g(is contained to lixisenatide 1139g) with purified water 100L dissolving, filter, collect filtrate for later use.

Purifying chromatographic condition:

High performance liquid chromatograph model: Novasep LC450

Chromatographic column: 450 × 250mm, in-built phenyl silane bonded silica gel is fixed phase stuffing, the particle diameter of this filler is 10 μ m.

Flow velocity: 5000ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 10% methyl alcohol/90% aqueous solution (v/v) of 30mM D-sodium tartrate and 30mM Sodium phosphate dibasic, adjusting pH with phosphoric acid is 2.5.

Mobile phase A phase process for preparation: take 2070g D-sodium tartrate and 1280g Sodium phosphate dibasic, appropriate purified water is crossed 0.45 μ m membrane filtration after dissolving, filtrate is all collected 300L container for storing liquid, adds purified water to 300L scale after adding 30L Chromatographic Pure Methanol, and adjusting pH with phosphoric acid is 2.5.Be finished and repeat preparation.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $80\%A+20\%B\stackrel{35\min }{\→}60\%A+40\%B$

Applied sample amount: 250.0g (6250ml).

Purge process: make chromatographic column balance loading after 5 minutes, operation gradient purifying, monitoring Fractional Collections object peak cut.Collected cut is carried out to purity detecting (chromatographic condition of purity detecting is identical with the condition of above-mentioned lixisenatide assay, measures with area normalization method), purity is more than or equal to 98% cut and removes after most of acetonitrile for turning salt; Purity is more than or equal to 70% and is less than after 98% cut is removed most of acetonitrile and reclaims and repeat this purge process, again collect purity and be more than or equal to 98% cut and remove after most of acetonitrile also for turning salt; Purity is less than 70% cut by liquid waste disposal.

Divide 16 sample introductions, repeat above operation.

Turn salt chromatographic condition:

High performance liquid chromatograph model: Novasep LC450

Chromatographic column: 450 × 250mm, in-built anti-phase C8 chromatograph packing material, the particle diameter of this filler is 10 μ m.

Flow velocity: 5000ml/ minute.

Detect wavelength: 280nm.

Mobile phase A phase: 0.2% Glacial acetic acid (v/v) solution.

Mobile phase B phase: trifluoroacetic acid aqueous solution.

Gradient: $95\%A+5\%B\stackrel{20\min }{\→}95\%A+5\%B\stackrel{2\min }{\→}85\%A+15\%B$ $\stackrel{20\min }{\→}65\%A+35\%B$

Loading volume: 2500ml.

Purge process: by chromatographic column balance loading after 5 minutes, operation gradient purifying, monitors and collect object peak cut.Object peak cut vacuum rotary steam is concentrated into freeze-drying after 9000ml.

After freeze-drying, obtain white powder solid essence peptide 704g.Purity 98.39%, each foreign matter content is all less than 0.5%.Purification yield 61.8%(is with lixisenatide cubage in crude product), total recovery 17.6%.

Claims (7)

1. a purification process for lixisenatide, it comprises the following steps:

Step 1: adopt high performance liquid chromatography to carry out purifying to the thick peptide of lixisenatide, described high performance liquid chromatography is take phenyl silane bonded silica gel as stationary phase, take the methyl alcohol/phosphate buffer soln of D-tartrate as mobile phase A phase, carry out mutually gradient elution take acetonitrile as Mobile phase B, the phosphatic volumetric molar concentration of mobile phase A in is mutually 20mM～50mM, and gradient is

Step 2: the cut that adopts high performance liquid chromatography to obtain step 1 turns salt purifying, described high performance liquid chromatography is take alkyl silane bonded silica gel as stationary phase, take glacial acetic acid solution as mobile phase A phase, carry out mutually gradient elution take acetonitrile as Mobile phase B, the concentration expressed in percentage by volume of the glacial acetic acid solution of mobile phase A in is mutually 0.1～0.3%; Step 3: collect solution freeze-drying.

2. the process of claim 1 wherein that the volumetric molar concentration of the mobile phase A of high performance liquid chromatography in the described step 1 D-tartrate in is mutually 10mM～50mM.

3. the process of claim 1 wherein that the mobile phase A of high performance liquid chromatography in the described step 1 D-tartrate in is mutually one or more D-tartrates that are selected from D-sodium tartrate, D-soluble tartrate, D-ammonium tartrate.

4. the process of claim 1 wherein that the mobile phase A of high performance liquid chromatography in described step 1 phosphoric acid salt in is mutually one or more phosphoric acid salt that are selected from sodium phosphate, Sodium phosphate dibasic, SODIUM PHOSPHATE, MONOBASIC, potassiumphosphate, dipotassium hydrogen phosphate, potassium primary phosphate, ammonium phosphate or primary ammonium phosphate.

5. the process of claim 1 wherein that the pH of the mobile phase A phase solution of high performance liquid chromatography is 2.0～4.0 in described step 1.

6. the process of claim 1 wherein that the volume ratio that the mobile phase A of high performance liquid chromatography in described step 1 methyl alcohol in mutually accounts for whole mobile phase A phase is 10～20%.

7. the process of claim 1 wherein that the stationary phase of high performance liquid chromatography is eight alkyl silane bonded silica gels, six alkyl silane bonded silica gels or tetraalkyl silane group silica gel in described step 2.